FastQCFastQC Report
Wed 25 May 2016
SRR1779679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496347
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG250595.048685697707451No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAT200.006949722352.57
GTATAGC601.2151568E-535.01
CGATAAC552.9910222E-431.81818415
CGTATCA1001.2572855E-831.49999856
CTATCGT450.004306126431.1111094
CGTAAAC450.004306126431.1111094
CTTATAG500.00720648527.99999834
TCGTAAA500.00720648527.9999983
TCGGTAG500.00720648527.9999985
AGGGATC750.001831570223.3333348
TACACCG1202.1648138E-623.3333325
ATAAGAG1253.086161E-622.43
GCTTAGG10500.022.070
CCAGTTA800.00266503321.87513
GTATAAG1304.336691E-621.5384641
AGGCTTA21650.021.50115470
GGCTTAG14900.020.67114370
GTATCAT1901.3502358E-820.26315957
TATTAAG1055.4605515E-420.02
GTCTTAG1055.4605515E-420.01
Produced by FastQC (version 0.11.5)