FastQCFastQC Report
Wed 25 May 2016
SRR1779679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496347
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT157903.181242155185787No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG20290.40878659486206226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC11570.23310305088980088No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16750.061.21110570
GTATGCC28600.051.439742
CCGTCTT28050.051.1995547
CGTATGC29250.051.00482641
TATGCCG29000.050.60939843
TCGTATG29950.050.6006440
CTTGAAA29650.050.5325957
CTCGTAT29350.050.44256639
TCTCGTA29950.050.24499538
TAAGACG352.075907E-550.03997843
GCCGTCT29100.049.8336346
GTCTTCT28850.049.6383749
GCTTGAA30150.049.4623556
TGCTTGA29100.049.45256855
TCTTCTG28900.049.43133550
TTGAAAA30650.049.34074858
TCTGCTT28850.049.27923653
CTGCTTG29050.048.69888754
ATCTCGT30700.048.5615337
CGTCTTC29650.048.5548148
Produced by FastQC (version 0.11.5)