FastQCFastQC Report
Wed 25 May 2016
SRR1779678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353050
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205645.824670726525988No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG200.006948068752.524
CATCGTT556.686034E-638.1818232
TCGTTGT601.2141645E-535.00000434
TACATAT400.002417171435.05
AGTTGCG400.002417171435.024
GTATAGC400.002417171435.01
CTTACCG400.002417171435.09
ATATTAG652.0999187E-532.30769351
TTGGACG450.004304458431.1111094
CTTTATA450.004304458431.11110937
TTGGGCG450.004304458431.1111092
TCTATAG450.004304458431.1111093
GCCGTAT703.4840672E-530.00000254
AACCACG952.8152317E-729.4736827
CGTATCA755.5772696E-528.056
GTAGACG500.007203710327.9999984
TACCTTG500.007203710327.99999814
TACCCCG808.653986E-526.255
TTCACTA808.653986E-526.2537
AGTTGGC808.653986E-526.2556