FastQCFastQC Report
Wed 25 May 2016
SRR1779678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353050
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT130733.7028749468913755No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG18170.5146579804560261No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC8640.24472454326582635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG13600.058.66139270
CGTATGC21200.054.3405641
TCGTATG21850.054.29579540
GTATGCC21300.054.1237942
TATGCCG21400.054.04228243
CTCGTAT21250.054.01729639
CCGTCTT20750.053.87733547
GCCGTCT21100.053.64800646
TCTCGTA21650.053.34257538
GTCTTCT21400.052.85095249
TCTTCTG21650.052.72587250
CGTCTTA200.006912376352.56840548
AGTTATA200.006955133352.48650737
ATGCCGT22000.052.40910344
ATCTCGT22100.052.4073437
TGAAAAA23050.052.38749359
CTTGAAA21950.052.1499957
TTGAAAA22500.051.9569258
CGTCTTC22050.051.8134548
CTGCTTG21850.051.28172354
Produced by FastQC (version 0.11.5)