FastQCFastQC Report
Wed 25 May 2016
SRR1779677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471949
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG272825.780709356307567No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCT150.002224089870.04
CCTTACG200.00694951252.53
CGTCTAA200.00694951252.59
CTCTCTA305.8758626E-446.66666455
GTGTATA651.0002623E-843.0769231
TAACTTA350.001255016840.059
GTATAAG753.5346602E-837.3333363
CGTATCA852.572051E-937.05882356
TGTATAA1003.255991E-1035.0000042
TAGTGCT501.7089587E-435.0000044
ATGTATG400.002417993735.01
GTCAGTT400.002417993735.043
AATACCC400.002417993735.03
ATCATTA904.498361E-934.99999659
TATCATT957.625204E-933.15789858
TCATTAA1001.2571036E-831.50000260
CCGTAAC450.004305913631.1111091
TATAAGA901.7549974E-731.1111094
CATTAAA1052.021261E-829.99999861
GTATCAT1052.021261E-829.99999857