FastQCFastQC Report
Wed 25 May 2016
SRR1779677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471949
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT169953.6010246869894837No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG24050.5095889598240487No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC11850.2510864521378369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTGA150.00221972570.03467651
GACTAAT150.00221972570.03467649
CCTTTAA150.002224396469.9975737
AGGGGGG17900.061.9679570
TGGGTAC200.006956294652.487053
ACCTAAT200.006956294652.487051
TGATTAA200.006956294652.4870532
TTAGTTA407.487615E-752.4870530
ATAGTGT200.006956294652.487058
TATAAGG200.006956294652.487054
CTAGAAA1150.051.7428157
TCGTATG32600.051.42458740
GTATGCC30750.051.14727842
CGTATGC31650.050.88868341
TAATCTC31400.050.81548735
CTCGTAT32250.050.7889339
TTGAAAA32850.050.5058858
TGAAAAA34100.050.50212559
GAAAAAA35500.050.47176760
CCGTCTT30000.050.45706647
Produced by FastQC (version 0.11.5)