FastQCFastQC Report
Wed 25 May 2016
SRR1779676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850691
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187892.208675065329244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC1253.5579433E-928.025
CATCGTA1403.947207E-1027.524
TACGCAC700.001226292825.03
GCCGTAT1151.4970628E-624.34782854
TCGTACT1301.6066042E-724.2307726
ACTGGTC1301.6066042E-724.230778
GTTCTAT952.7805837E-422.1052631
ACGGTTT1154.11789E-521.3043512
ACATCGT1656.794653E-821.21212223
TCGTTTA850.00378996720.58823632
TTCCGTT1205.7154546E-520.41666621
AGGCTTA21000.019.570
ATTCGTA900.005275759419.4444437
TCGTTGT1107.474938E-419.09090834
TCCGTTG1107.474938E-419.09090822
ACGGTTA1107.474938E-419.09090850
GTACAAG1852.2702443E-718.9189191
GTTCCGT1301.0572256E-418.84615520
TACGAAG950.00720910218.42105164
CATCGTT950.00720910218.42105132
Produced by FastQC (version 0.11.5)