FastQCFastQC Report
Wed 25 May 2016
SRR1779676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences850691
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT110031.293419114578619No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG14700.1728006996665064No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC8890.10450328027450624No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG20100.051.01677340
CGTATGC19650.050.9743141
CCGTCTT18500.050.9456847
CTCGTAT19850.050.59250639
GCCGTCT19400.049.30463446
TATGCCG19700.049.08157743
TCTCGTA20850.049.0051438
GTATGCC19750.048.7799442
CGTCTTC19800.048.1316248
ATCTCGT21100.047.59532537
CTTGAAA21350.047.38807357
GTCTTCT20550.046.68301449
ATGCCGT21050.045.767444
TCTTCTG21100.045.3002350
AGATCTC23000.044.728535
CTGCTTG21100.044.48624854
TTGAAAA23550.044.29644858
CGTATGA950.044.2390341
AGGGGGG15100.043.7975470
TGCCGTC22100.043.27844245
Produced by FastQC (version 0.11.5)