FastQCFastQC Report
Wed 25 May 2016
SRR1779675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363960
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG164274.51340806682053No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTA400.002417594234.9990468
CGTATCA751.486851E-632.67025862
GTATCAT952.8129398E-729.47692563
TATTGAC604.978484E-429.1658657
GTAACAA500.00720496327.9992313
ACACACG657.9501077E-426.9223388
ATCATTA1056.756927E-726.66593465
AGTTTCC700.001225140824.99931117
TAGACAC1052.0361009E-523.3326935
CCTCTAT750.001830928623.33269366
CATTAAA1202.162691E-623.33269167
GACGTAG1253.0831452E-622.3993851
ACGTAGA1253.0831452E-622.3993852
CATACTC1154.1104417E-521.3037645
CGAAGAC1154.1104417E-521.3037643
GCCGTAT1003.9235957E-421.0023160
TCATTAA1356.007471E-620.74017166
TATGTAA850.003783452620.59049858
TCGATGG850.00378646620.5876770
TGACCCA850.00378646620.5876710
Produced by FastQC (version 0.11.5)