FastQCFastQC Report
Wed 25 May 2016
SRR1779675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363960
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT80982.2249697768985603No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG11550.31734256511704584No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5270.14479613144301573No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT3800.10440707770084624No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT3660.10056050115397296No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG150.002225064769.989015
ATCGTAT308.37643E-658.32417739
TACGTAC252.3907129E-455.99120710
CGAGCTA200.006952556352.4917564
TATCGTA407.478193E-752.49175638
CATATGC950.051.60629751
TCGTATG14300.051.14581740
CGTATGC13550.050.12349341
GTATGCC14300.049.7116642
CCGTCTT10600.049.56819547
CTCGTAT14950.049.3902439
TCTCGTA15400.048.62872738
TATGCCG14450.048.4752643
ATCTAGT1600.048.11744337
AGGGGGG7900.047.84058870
TGAAAAA17500.047.39255559
GCCGTCT11250.047.01561746
CGTATGA1200.046.67857741
TTGAAAA13700.046.48904858
CGTCTTC9800.046.46596548
Produced by FastQC (version 0.11.5)