FastQCFastQC Report
Wed 25 May 2016
SRR1779674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418281
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284956.812406014138821No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAT200.006949617552.4987566
CTATACG352.0839985E-550.0047844
TAGACCG552.988546E-431.8212265
GACTTTA552.990643E-431.81742140
TAAAACG450.004303342731.1140884
CATAGTT450.004303342731.1140884
TCATTAA1800.031.1103760
ATTTCGG604.9796334E-429.16597230
TTCGGGA755.5760607E-528.002682
TACCGGA500.007201845328.0026782
GTTACCG500.0072060527.9993357
ATCATTA2000.027.9993359
GCCGTAT1800.027.22157554
GTACAAG657.946386E-426.9256521
CTATATC657.946386E-426.9256524
TATCCGG657.946386E-426.9256525
TTATACT1358.049028E-925.9284064
CGTATCA1900.025.78885856
GTATCAT1900.025.78885857
TCCCTTT700.001224567125.00239222
Produced by FastQC (version 0.11.5)