FastQCFastQC Report
Wed 25 May 2016
SRR1779674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418281
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT177254.237581912637676No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG24410.5835789815937134No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC14220.33996284794193377No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6650.15898403226539098No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT4790.11451631797762749No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTACC150.002225750869.9854134
AGGGGGG17150.061.61981270
CTCGTAA1500.056.0084239
AGATAAT900.054.43310532
TCGTATG31900.052.5627640
CTTGAAA29800.052.50789357
ATAAGTC200.0069448352.50789339
CGTATGC29900.052.4681841
TCTTCTG25800.052.10708650
TCTCGTA33000.052.0835938
CGTCTTA1750.052.04516248
CTCGTAT32250.051.99231339
CTTCTGC26000.051.97556751
GTATGCC30050.051.86288542
TATGCCG29650.051.85384443
GCTTGAA30050.051.5009256
GTATGAC2450.051.46092242
TTCTGCT24800.051.38483452
TCTGCTT26000.051.31458353
CCGTCTT27200.051.25158347
Produced by FastQC (version 0.11.5)