FastQCFastQC Report
Wed 25 May 2016
SRR1779673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441192
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG234375.312199677238027No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTA350.001254927740.00000415
TATCGGT501.7087781E-435.00000424
CGTATCA806.236223E-835.056
CGTTGGA400.00241782335.024
AGTCATC604.9793744E-429.1666668
TCTACGT604.9793744E-429.1666662
GCCGTAT853.951727E-628.8235354
CGCATTT500.007205627428.00000238
CTTGTGC657.9515204E-426.9230791
TACTGTT800.002664567721.8757
GTTAAGC850.00378711720.5882363
GTGTATA850.00378711720.5882361
TATCGCG850.00378711720.58823611
GCTCCAA850.00378711720.58823639
TTGTACT900.00527181319.4444454
AGAACGT900.00527181319.44444541
TTAAGCT2002.4319888E-819.2500024
TGTATGC1107.46714E-419.0909089
GTAGATC1301.0557594E-418.84615538
TATAAGA1301.0557594E-418.8461552
Produced by FastQC (version 0.11.5)