FastQCFastQC Report
Wed 25 May 2016
SRR1779673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441192
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT145693.302190429563546No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG20250.45898384376869933No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC10560.23935157482456618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG15050.056.97434670
ATCGTAT509.822543E-1055.99129539
TCGTATG26200.053.29324340
CGTATGC25700.053.25885841
TATGCCG25300.053.14438643
CCGTCTT23900.053.046447
CTCGTAT26300.052.5583739
GTATGCC25900.052.18362842
CTTGAAA25700.052.1514357
TCTCGTA26650.052.13073338
TTGAAAA26700.051.90204258
GCCGTCT24950.051.37546546
GTCTTCT24550.051.35659449
ATGCCGT26300.051.1294944
ATCTCGT27250.051.1113237
CTGCTTG24100.050.8565654
CGTCTTC25250.050.77082448
GCTTGAA26300.050.56821456
TGCTTGA24900.050.33628555
TCTTCTG24850.050.31379350
Produced by FastQC (version 0.11.5)