FastQCFastQC Report
Wed 25 May 2016
SRR1779672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145191
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2822719.441287683120855No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.188028183565097No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2190.15083579560716573No Hit
CTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTAGATCTCGGTG1930.13292834955334695No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAAAA150.002220979270.0000164
CGCGATT150.002220979270.0000135
TAGCTTA150.002220979270.000019
ATTTGGA150.002220979270.0000135
AGAGATA150.002220979270.0000130
CTTGTGC252.3840593E-456.0000041
TTGTGCG252.3840593E-456.0000042
TGTGGGC252.3840593E-456.0000043
CTATGGC200.006939866652.551
GGGCTAT200.006939866652.548
CTTCCGA200.006939866652.562
TAGGTCG200.006939866652.56
TATGGCG200.006939866652.552
GCTATGG200.006939866652.550
TTCCGCG200.006939866652.550
GCGATTC200.006939866652.536
GATTCGT200.006939866652.538
TTTTACC200.006939866652.540
GTTGTGG451.6755566E-646.6666681
TACGAGA305.862348E-446.66666814
Produced by FastQC (version 0.11.5)