FastQCFastQC Report
Wed 25 May 2016
SRR1779672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145191
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1804912.431211300975956No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG25861.7811021344298201No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC11960.8237425184756632No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4070.2803204055347783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3400.23417429454993766No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3250.22384307567273454No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT3130.21557810057097204No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2920.20111439414288768No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2500.17218698128671886TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT2390.16461075411010323No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT2290.1577232748586345No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2120.14601456013113762No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT1610.11088841594864694No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT1560.10744467632291257No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1550.1067559283977657No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTAA150.002218508270.0196455
GTCTTAA150.002218508270.0196449
AGACTAA207.9078454E-569.9955225
TTCAGTT150.00222154369.9955230
GATTTTC150.00222154369.9955226
CGACATA150.00222154369.9955214
ACTAATT207.9078454E-569.9955227
TCCAAAT150.00222154369.995527
CTTTACA353.6852725E-969.995521
CTCTATA150.00222154369.995521
TCAGTTG150.00222154369.9955231
AGGGGGG17750.061.91153370
TATGCAG750.060.6836943
TCGTAAG750.060.66278540
CCGTATT352.9520197E-760.01683447
GCCGTAT352.9520197E-760.01683446
ATACTTG308.34658E-658.329628
AGAATAC308.34658E-658.329625
CTCGTAA800.056.87135739
TTAAGCT252.3807547E-456.01571752
Produced by FastQC (version 0.11.5)