FastQCFastQC Report
Wed 25 May 2016
SRR1779671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222365
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2930813.18013176534077No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2730.12277111955568548No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570.11557574258538889No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTAAA353.6961865E-970.0000161
CTTTACG150.00222253970.02
TCATTAA401.061926E-861.2560
ATTAAAA509.767973E-1055.99999662
TCCTAGC200.006944701552.565
GTGCTTT200.006944701552.533
GTATCTA200.006944701552.547
GACATAA200.006944701552.514
ATCATTA407.45682E-752.559
TATGTGC200.006944701552.53
TAATGCA200.006944701552.52
TATCATT352.0819954E-550.00000458
AATGCAC305.8691227E-446.6666643
TAGATCT551.3062345E-744.54545639
TATAAGA404.5828005E-543.752
AATATAA350.001253585840.00000443
ATAAGAG350.001253585840.0000043
GTATAAG350.001253585840.0000041
CGTATCA556.6748726E-638.1818256
GCCGTAT601.2121471E-535.054