FastQCFastQC Report
Wed 25 May 2016
SRR1779671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222365
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT175597.896476513839858TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG23661.0640163694826075TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC12380.5567422930766982RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT4130.1857306680457806TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3910.1758370247116228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3700.16639309243810851No Hit
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3430.15425089380073304TruSeq Adapter, Index 5 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3300.14840465001236705TruSeq Adapter, Index 5 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT2560.11512603152474535TruSeq Adapter, Index 5 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT2480.11152834303959705No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2410.1083803656150923RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT2320.10433296606930048No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGTT150.002210406170.0966442
TCTACTG301.0155236E-770.049350
TGTAATG150.002224266569.986285
ACTAAGA150.002226251969.970548
TATGTCA150.002226251969.9705461
ACTCGTA501.4551915E-1162.9876638
AGGGGGG15350.062.44927670
GTAAGCA252.3706062E-456.0773242
GTCATAT252.378536E-456.0394449
CTCGTAA950.055.26475539
TCGTAAG950.055.26475540
CGTATGA1350.054.5196141
CTTTACA653.6379788E-1253.8598251
TCGTATG29700.053.85677740
CTCGTAT29200.053.58031539
TTCTGCT24600.053.53361552
GTCTTCT25200.053.37089549
CTTGAAA28750.053.32328457
CGTATGC29250.053.32137741
TCTCGTA29750.053.2836738
Produced by FastQC (version 0.11.5)