FastQCFastQC Report
Wed 25 May 2016
SRR1779670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609743
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152192.4959696134272966No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT200.00695070452.4995731
AGCGTAA450.004305335731.11340758
CGTAACT450.004305335731.11340760
TATTGGA604.9818197E-429.16642836
GCCGTAT1900.027.63135354
TCTTGGA906.1697247E-627.2219962
GTTATTG657.955407E-426.92285534
ATTGGAT700.001225952924.99979437
CGTATCA2250.024.88868356
ACTCAAT851.3081077E-424.7056840
CCGTATC2450.024.28551555
GATCCAA1052.0387213E-523.33314348
ATAACTT1052.0387213E-523.33314354
ACATACC901.9275352E-423.333143
GTATCAT2155.456968E-1222.79238157
ACTCCTT16000.022.31231770
CAAGTTA1102.9207387E-522.27254531
AGTACGA800.002665851321.8748219
ATTTCGT800.002665851321.874826
TAGATCT2750.021.63618539
Produced by FastQC (version 0.11.5)