FastQCFastQC Report
Wed 25 May 2016
SRR1779670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences609743
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT83711.3728734893225507No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG16070.2635536611326411No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8910.14612713881094166No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT8420.13809096619395383No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC6730.11037437084148569No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG14800.051.08287440
CTCGTAT14200.050.7722739
CGTATGC14550.050.29318241
AGGGGGG9500.050.09880470
GTATGCC14200.049.8109142
CTTGAAA15500.048.99770457
CCGTCTT14800.048.50928547
TCTCGTA14950.048.45529638
GTCTTCT15600.047.8136749
TTGAAAA16100.047.60256258
TTCTGCT15600.046.6874552
TCTGCTT15800.046.31809253
TATGCCG15600.045.78961643
GCCGTCT15600.045.56889746
GCTTGAA16750.045.1322156
TGAAAAA17450.044.92257759
TCTTCTG16450.044.917350
AATCTCG16600.044.69303536
ATCTCGT16350.044.52025237
TGCTTGA16700.044.22673855