FastQCFastQC Report
Wed 25 May 2016
SRR1779669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275561
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185146.718657574910818No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG5870.21301998468578642No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCGC200.006946457552.544
CTATTCG200.006946457552.543
GCCGTAT1200.046.66666454
CGTATCA1250.044.856
CATTAAA1450.043.44827761
TCATTAA1650.040.30303260
TAGATCT1400.040.039
CAAGACT350.001254108340.06
GTAGATC1500.039.66666838
GTATCAT1500.039.66666857
CCGTATC1450.038.6206955
ATCATTA1700.037.05882359
ATCTCTA400.002416253335.040
GTTTACA501.707117E-435.01
TACAAGA601.2131988E-535.04
TCTCGGT1750.034.043
AGATCTC1950.032.30769340
TATCATT2000.031.49999858
CTGTCAG450.004302833731.1111139
ATCAGCT450.004302833731.11111320