FastQCFastQC Report
Wed 25 May 2016
SRR1779669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275561
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT100233.6373071661084118No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG17990.6528500041733046No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT13010.47212776844328475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10780.3912019480260269No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC8870.3218888013906177No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3720.13499733271399073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA301.01526894E-770.0608548
CGTAACT150.00222504869.9845834
TAATCGA252.3904386E-455.9876621
AGGGGGG11200.054.9878870
ACCGATG200.006942539552.50748412
ACTTGTA200.00695251452.488437
CGATTGC200.00695251452.4884333
GACGCTT200.00695251452.4884320
ACGTAAC200.00695251452.4884333
TAACTCG200.00695251452.4884336
TATGCCG20800.050.6930743
CGTATGC21000.050.68309441
TCGTATG21750.050.36585640
GTATGCC21200.050.0579842
CTCGTAA352.083786E-549.99805539
AGACTTC352.0860247E-549.9889836
CTTGAAA20300.049.98898357
ATTATAC3300.049.83751
CTCGTAT21650.049.79021539
CCGTCTT20400.049.4547247
Produced by FastQC (version 0.11.5)