FastQCFastQC Report
Wed 25 May 2016
SRR1779668_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779668_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312639
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG183325.863631856550207No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG4790.1532118513685113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACTG150.002223385370.05
ATGTAAA150.002223385370.039
GTGTATA301.0218537E-770.01
TGTATAA352.968918E-760.02
ATCATTA1100.054.09090859
TAGCGTG200.006947327452.55
CGTGTAT200.006947327452.563
TCATTAA1150.051.73913260
CGTATCA1400.050.056
TATCATT1200.049.58333258
CATTAAA1300.048.46153661
TGTAAAA305.8728014E-446.66666440
GTATCAT1600.043.7557
TTAAAAA1450.041.0344863
AAAGGTG350.00125436740.016
GTAAAAT350.00125436740.041
ATTAAAA1600.039.37562
GTATAAG556.6835764E-638.1818163
CCGTATC1750.038.055
TAGATCT1900.036.84210239
Produced by FastQC (version 0.11.5)