FastQCFastQC Report
Wed 25 May 2016
SRR1779668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312639
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT100323.2088127201020984No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG18920.6051708200192555No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT12750.4078186022857033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10510.3361704713743327No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC8910.284993235009068No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT3660.11706792818554307No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT207.905177E-570.02576452
TAAGACG150.00222437369.9921654
AGGGGGG10850.064.1863670
ATTATAC3050.058.5180361
TCTTTAT308.37144E-658.32681
CTAGCGG200.00695040352.49412523
GTATGCC20800.052.0060142
CGTATGC22150.051.9889541
GTATGCA1550.051.9462942
TGAAAAA23050.051.6293759
TCGTATG22600.051.57327340
GAAAAAA23650.051.50353260
CTCGTAT21750.051.48848739
TCTGCTT20900.051.43040553
TTGAAAA21850.051.4212358
AATATAC750.051.3275831
CTTGAAA21550.051.32496657
GTCTTCT22250.050.8276949
CCGTCTT20650.050.53515247
GTAGATA1250.050.39435633
Produced by FastQC (version 0.11.5)