FastQCFastQC Report
Wed 25 May 2016
SRR1779667_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779667_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321564
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG165075.133348260377405No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG5450.1694841462352751No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA150.002223443570.029
ATTGCGT252.3884389E-456.0000048
TACGGTT200.006947507652.548
TGCGTTA352.084092E-550.010
CGTATCA950.047.8947456
AGTCTTA305.8730535E-446.66666415
GCCGTAT1250.036.39999854
GTATCAT1350.036.29629557
GCTAATC400.002416851735.08
TCGGTGG1750.032.045
TGGTCGC1750.032.049
CACTCGC450.00430389231.11111341
TCTCGGT1800.031.11111343
ATAAGTC450.00430389231.11111318
CCGAAAT450.00430389231.11111347
CCGTATC1357.2759576E-1231.1111155
TAGATCT2050.030.73170939
CGCCGTA1650.029.69697253
GTTCTGA604.9764075E-429.1666661
GTAGATC1950.028.71794938
Produced by FastQC (version 0.11.5)