FastQCFastQC Report
Wed 25 May 2016
SRR1779667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321564
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT91662.850443457600975No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG15720.4888606933611972No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT11160.34705377467626974No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10410.3237302683136172No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC8490.2640220920252267No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3260.10137950765632968No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG11700.058.32633270
TATGCCG18750.051.72459443
TCGTATG20350.051.59086240
CTCGTAT20100.051.53610239
CGTATGC19700.051.53269241
ATCTCGT20350.051.0749537
CGTATGA1100.050.9188241
GTATGCC19250.050.74485842
CCGTCTT18500.050.73606547
TCTCGTA20450.050.6540738
ATGCCGT19100.050.5934544
GCCGTCT19000.050.49904346
CGTCTTC19350.050.31732648
GTCTTCT19600.050.1959749
TATGACG1050.050.01733443
TGCCGTC19400.049.81107345
GCTTGAA20450.049.6427456
CTTGAAA20450.049.63501757
TCTTCTG19900.049.43924350
CTTCTGC20500.049.3585751