FastQCFastQC Report
Wed 25 May 2016
SRR1779666_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779666_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318881
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140154.395056463069295No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG4700.1473904058253706No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGAA150.002222181870.0098858
CGACTAG200.00694788552.49917641
CGTATCA1550.042.90255456
ATCATTA1600.037.1927559
GTATCAT1700.035.00494457
CGCGCTA400.00241514835.0049459
CCGTATC1800.034.9994555
CAGTAGT601.2139268E-534.999454
TACACTT400.002417007234.999455
GCCGTAT1800.034.9994554
TCATTAA1900.031.3202160
GTGTACC450.004304169731.11062220
CATTAAA1950.030.51712661
TAGATCT2000.029.74953339
AGCCGTG604.9767713E-429.1662122
TAGCTTG500.0072032327.99956145
GTACGAG500.0072032327.9995611
AGATCTC2300.027.39087340
ACTATTC657.947388E-426.9226558
CCCAACG657.947388E-426.92265553