FastQCFastQC Report
Wed 25 May 2016
SRR1779666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318881
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT78322.4560886349453246No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG13930.4368400751377473No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT8860.27784659481123053No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8130.2549540424170772No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7090.22233999517061223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG8650.056.23206370
CCGTCTT14900.053.6056947
TCGTATG15500.053.05441340
GTATGCC15000.053.0147742
CTCGTAT15050.053.0131639
CGTATGC14950.052.94113541
TATGCCG15100.052.6636843
ATGCGTA200.00693115152.53119341
TTGAAAA15800.052.49823458
GCGGTAA200.00695268852.4913
TCTCGTA15400.052.03554538
GCCGTCT15400.051.63776846
TCTTCTG15750.051.5780950
TATGACG750.051.3799643
ATTATAC2250.051.323561
CTTGAAA16100.051.08524357
CGTCTTC16000.050.79609348
TGAAAAA16550.050.75358259
GCTTGAA16000.050.74829556
ATGCCGT15700.050.6510644
Produced by FastQC (version 0.11.5)