FastQCFastQC Report
Wed 25 May 2016
SRR1779665_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779665_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508997
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202553.9793947704996295No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG7060.13870415739189032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT150.002224189770.0000129
CGCGTGT200.00694982352.57
GCCGTAT1250.039.254
GAACGTA400.002418171135.06
CTCGACT400.002418171135.011
CGCCGTA1750.030.053
TCTCGGT2100.029.99999843
CGTATCA1650.029.6969756
TCGGTGG2300.028.91304645
TGGTCGC2350.028.29787349
CCAACGT500.007206653228.00000222
CGAAGTG500.007206653228.0000025
GTGGTCG2300.027.39130648
CGGTGGT2550.026.07843246
CCGTATC1900.025.78947355
TGAGCGT700.001225615925.022
GTAGATC2600.024.23076838
TCGCCGT2350.023.82978852
CTCGGTG2500.023.844
CGTGCGA750.00183163423.33333410
Produced by FastQC (version 0.11.5)