FastQCFastQC Report
Wed 25 May 2016
SRR1779665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508997
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT111282.186260429825716No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG20410.40098468163859513No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13520.26562042605359165No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT11750.23084615429953417No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC10090.19823299547934467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG14800.052.2497270
TATGCCG23550.050.13574243
TCGTATG25050.049.8817840
CGTATGC24450.049.8370841
CTCGTAT24500.049.5680939
GCCGTCT23550.049.55040446
ATGCCGT23950.049.44468744
CCGTCTT23050.049.4090347
CGTCTTC23950.049.308148
TCTCGTA24700.049.16189638
CGTATGA1000.049.02078241
GTATGCC24100.048.98192642
ATCTCGT24850.048.58350437
TGCCGTC24250.048.55359645
CTTGAAA25150.047.73507357
AATCTCG25250.047.67058636
CTGCTTG24700.047.35266554
GTCTTCT25400.046.88409449
CTTCTGC25650.046.01748351
TGCTTGA25350.045.99130655