FastQCFastQC Report
Wed 25 May 2016
SRR1779664_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779664_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447462
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG192934.311651045228421No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG5080.11352919354045707No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATATA200.00694651452.505285
TTACCAC200.006949584552.4994133
ATACGTT305.8758573E-446.66614531
AAGTATC400.002417992834.99960745
TCATTAA851.0626354E-732.94080760
TATGGCA552.9907518E-431.8178252
TCTATAC552.9907518E-431.8178253
ATTAAAA1001.2569217E-831.49964762
CCCTTTG450.00430591331.11076414
TATCATT1052.0208972E-829.99966458
TATACCG604.9765554E-429.16965
CTAAATA500.007206132627.99968739
TAGATCT1403.947207E-1027.49969339
CTATACC906.165057E-627.2219184
ATCATTA959.381931E-625.78918659
CATTAAA1101.0146814E-625.4542661
AGAGTGC700.001224665425.0025148
TATACCA851.3063828E-424.7083665
ATACCGA1001.3951942E-524.5024626
GAGAGTG750.001830218123.3356827
Produced by FastQC (version 0.11.5)