FastQCFastQC Report
Wed 25 May 2016
SRR1779664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences447462
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT111642.494960465916659No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG18480.41299596390308No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT12830.28672825848898903No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10020.22392962977861808No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC7940.1774452355730766No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTAG150.00222578969.985923
AGTACTT150.00222578969.985925
AGGGGGG12050.059.2411970
ATTATAC2900.054.299421
TCGTATG21700.052.89258640
TTGAAAA21950.052.62076658
ACTAGGC200.00695480852.4894429
GTATGCC21450.052.4080842
CCGTCTT20650.052.25127847
CGTATGC21800.052.20340741
TGAAAAA22600.052.185659
CTTGAAA21750.052.139157
CTCGTAT21850.051.8888739
GAAAAAA23750.051.57433360
TCTCGTA22350.051.51088738
GTCTTCT21250.051.42386649
TCTGCTT21750.051.20217553
TCTTCTG21600.050.9149150
TGCTTGA22000.050.7681655
GCTTGAA22300.050.69619856
Produced by FastQC (version 0.11.5)