FastQCFastQC Report
Wed 25 May 2016
SRR1779663_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779663_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682116
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180762.649989151405333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA604.9821933E-429.1666661
CGTATCA1950.026.92307756
GTATACC700.001226043625.01
GCCGTAT2250.023.33333454
CGGTAAG750.001832270923.33333228
GGAGTTA800.002666045421.8750026
ACGCTTT800.002666045421.87500217
GTATCAT2451.8189894E-1221.42857257
CCGTATC2501.8189894E-1221.055
ATCATTA2501.8189894E-1221.059
TTCGGTA850.00378920820.58823626
TATCTTG1055.4634316E-420.044
TAATGGT1055.4634316E-420.016
GTGACAC900.0052747119.44444349
ACGACTC900.0052747119.4444438
ACTCCTT12800.019.41406470
TAGATCT2757.2759576E-1219.0909139
ACTGGAC1301.0568352E-418.8461553
CGCCGTA2659.458745E-1118.49056653
CTAGAGT950.007207670718.4210533
Produced by FastQC (version 0.11.5)