FastQCFastQC Report
Wed 25 May 2016
SRR1779663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682116
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT83711.2272106210673845No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG13600.19937957766714165No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10150.14880167009716821No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT9310.13648704912360948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG16050.053.4145440
CTCGTAT16300.053.02464739
CGTATGC15450.052.12217341
TCTCGTA17050.051.92357338
GTATGCC16300.051.78594642
TATGCCG16500.051.1582443
CCGTCTT13700.050.89117447
GCCGTCT14150.050.26313446
GTCTTCT12000.049.0390949
CGTCTTA1750.048.0488748
ATCTCGT18500.047.8538937
CGTCTTC14100.047.21113648
TCTTCTG12300.046.98867450
TGAAAAA21200.046.87947559
CTTGAAA16000.046.80862457
ATGCCGT18150.046.70389644
CGGATCT21400.046.27443334
TTGAAAA16900.045.76544658
CGTATGA1150.045.66848441
AGGGGGG9900.044.8886970
Produced by FastQC (version 0.11.5)