FastQCFastQC Report
Wed 25 May 2016
SRR1779662_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779662_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331939
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184145.547404794254366No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG11810.35578826230120597No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGACC150.002223506670.043
TAGATCT2100.041.66666839
CCGTATC1950.039.4871855
GTATCAT1950.039.4871857
GCCGTAT2050.039.2682954
CGTATCA2000.038.556
ATGGGAC501.7078688E-435.03
GAATGGT400.00241696335.025
TAAGACT400.00241696335.09
GTTATAC400.00241696335.01
GTAGATC2650.034.33962238
ATCATTA2150.034.18604759
AGATCTC2800.032.540
CATTAAA2300.031.9565261
TACCCAG450.004304090531.1111095
ACTCTTG450.004304090531.1111095
CGTCTAT450.004304090531.1111091
TTAAGCA450.004304090531.1111092
CGAATCA450.004304090531.11110956
CTCTGGA450.004304090531.1111092