FastQCFastQC Report
Wed 25 May 2016
SRR1779662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331939
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT102163.077673909965385No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG19910.599809001051398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19640.5916749764263916No Hit
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT11200.33741139185211744No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC8190.24673208029186086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTAG150.00222403869.995781
AGGGGGG9550.054.9622370
CGTCTTA1100.054.14475648
TATCACG200.00695350152.48892636
TATAAAG200.00695350152.48892631
CCGTCTT17700.051.6615547
CTTGAAA18650.051.4255657
TCGTATG20350.051.41421540
CGTATGC19700.051.37304341
GTATGCC19450.051.33640342
TATGCCG19450.051.15627343
CTCGTAT20450.050.8205739
TCTCGTA20750.050.2544638
TTGAAAA19750.049.979258
GTCTTCT17050.049.9022549
TTCTGCT16500.049.86050852
GCTTGAA19100.049.8474456
TCTGCTT17150.049.8229453
TCTTCTG16900.049.7236250
TGAAAAA21700.049.6819159
Produced by FastQC (version 0.11.5)