FastQCFastQC Report
Wed 25 May 2016
SRR1779661_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779661_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461153
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG193114.187547299919983No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG6620.1435532242010786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1950.041.28160556
GCCGTAT2100.039.9995754
CCGTATC2150.039.06934455
CGTAATA400.00241806534.99962231
CGGCTTA501.7090075E-434.99962225
AGATCTC2850.031.92947840
TAGATCT2750.031.81783739
TCGGTGG2750.031.81783745
GTATCAT2450.031.43163757
GTTATTC450.00430604131.11077311
AGAGTCC703.4866174E-529.9996766
ATCATTA2700.029.81772459
CGCTTAC604.980036E-429.1663511
TCATTAA2650.029.0594460
GTAGATC3100.028.225538
TCTCGGT3100.028.225543
ACTGCGT500.00720634427.9996998
CCTAAGT500.00720634427.9996992
TTCGGTA500.00720634427.99969926
TCTATGG500.00720634427.9996992
Produced by FastQC (version 0.11.5)