FastQCFastQC Report
Wed 25 May 2016
SRR1779661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461153
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT108752.358219506324365No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG18590.4031200057247811No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12540.2719271044534027No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT12410.2691080834343483No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC8830.19147658152500363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG308.378856E-658.32547440
AGGGGGG11500.055.68814570
CGTATGC23000.050.52588741
TTGAAAA22950.050.3200258
TCGTATG24000.050.3057240
CCGTCTT21250.050.27213347
CTTGAAA22700.049.94921557
GTATGCC23000.049.93338842
CTCGTAT24150.049.84835439
TATGCCG23050.049.67316443
TCTCGTA24650.048.979238
CGTCTTC22100.048.8140548
GTCTTCT21850.048.56051649
TCTGCTT21650.048.51861653
TCTTCTG21800.048.5112650
GCCGTCT22250.048.48496646
ATAGCAT25550.048.34964832
AGCATCT25350.048.17891334
TGAAAAA25650.048.1611559
ATGCCGT23950.047.9579344
Produced by FastQC (version 0.11.5)