FastQCFastQC Report
Wed 25 May 2016
SRR1779660_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779660_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148629
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1700911.44393086140659No Hit
CTTATACACATCTGACGCTGCCGACGATACTCCTTGTGTAGATCTCGGTG4810.3236245954692557No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1760.11841565239623492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTAC200.006940189752.50000413
GTTTATA200.006940189752.50000448
CCTACAG200.006940189752.50000428
GGAACTA200.006940189752.50000456
AACCTTA200.006940189752.50000460
CAATGTG200.006940189752.50000414
TTGATGT404.5754234E-543.75000411
CTTGATG404.5754234E-543.75000410
CTTGTGG350.00125224440.01
GGTACCG350.00125224440.05
CGCTACT350.00125224440.067
TGATGTA459.163277E-538.88888512
GCCGTAT753.5068297E-837.33333654
CGTATCA806.188748E-835.00000456
TCCACAA400.00241268335.00000455
TTGCACT400.00241268335.00000429
AGATCTC904.454705E-934.99999640
CCGTATC851.0544136E-732.94117455
TAGATCT851.0544136E-732.94117439
GGTGCCA450.00429651631.11110910