FastQCFastQC Report
Wed 25 May 2016
SRR1779660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148629
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT95336.413956899393793No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG17321.1653176701720391No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT12170.8188173236716926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8850.5954423430151585No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC6720.45213249096744246No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3100.20857302410700468No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2670.17964192721474276No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT1890.12716226308459316TruSeq Adapter, Index 12 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACT401.3278623E-1070.0949
GTAATCT150.002209788770.0899949
ATCTAGT150.002224601669.9720837
ACGTATT150.002224601669.9720835
AAACTCG252.8355425E-669.9720736
CGTAAGC550.063.6752541
AGGGGGG9350.063.2367970
TCGTAAG501.4551915E-1162.9960640
CTCGTAA453.8198777E-1062.2183339
GAAAACT352.966408E-759.97606334
ATTATAC2750.059.794321
CGTCTTA308.264482E-658.42801748
AGTATGC308.314191E-658.3689841
ATATCGT308.364146E-658.31006237
CTGAGGA252.3889505E-455.97765723
AATCGTA252.3889505E-455.97765738
CGTATGC19450.054.5577441
TATGCCG18950.054.5555443
GTATGCC19050.054.45311742
CCGTCTT18350.054.44790647