SRR1779659_1.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1779659_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1573
Sequences flagged as poor quality	0
Sequence length	76
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	162	10.298792116973935	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG	36	2.2886204704386524	TruSeq Adapter, Index 5 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	13	0.8264462809917356	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	10	0.6357279084551812	RNA PCR Primer, Index 5 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	9	0.5721551176096631	No Hit
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	5	0.3178639542275906	TruSeq Adapter, Index 5 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	5	0.3178639542275906	RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT	4	0.25429116338207247	TruSeq Adapter, Index 5 (95% over 23bp)
AACTAATCCCTAACTGGTGGGATAGGGGTACAAGCTGTCTCTTATACACA	3	0.19071837253655435	No Hit
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT	2	0.12714558169103624	No Hit
CAGCTGTGTTCCTCTACTCTTGGCCTGGGCCCTCTCCTATCACTACAGTG	2	0.12714558169103624	No Hit
GTGTTCACCCGACTCAGGCTAGCGCGGGACCGCGCCGGCGCCTCCCCCCT	2	0.12714558169103624	No Hit
GGTTGACGATCTGGATGTTGAGCACCGCCATGAAGGCGCCGAGCGTGGCG	2	0.12714558169103624	No Hit
GTCCCGGACCGGCGTCCCGCGGCGGGGGCACGACCTGTCTCTTATACACA	2	0.12714558169103624	No Hit
GGGCCCACGGCGTCCGGGACGCGCTGGCGCACGGGGTGAAGGTCACCGGC	2	0.12714558169103624	No Hit
ATCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC	2	0.12714558169103624	TruSeq Adapter, Index 5 (95% over 21bp)
TCATCGGCGCGCTGGCGTGGGTGACGGAGAAGGTGCTGCTGCGGCACGTG	2	0.12714558169103624	No Hit
AGCCCGGCCTCGACGACGGCGGCGTCCGCCTCGTGGATCAGGTGGCCGCT	2	0.12714558169103624	No Hit
CGCGCCGCGCGCATCGTCGGAACGGTGCTGGCGTCCCTGTCCGTGCTGGT	2	0.12714558169103624	No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT	2	0.12714558169103624	No Hit
CATAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	2	0.12714558169103624	No Hit
CTTATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT	2	0.12714558169103624	No Hit
GATCCGAGCTGATGACGGGAATGTGACGCGGCCGGGACAACGACGGCGCC	2	0.12714558169103624	No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCG	2	0.12714558169103624	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	25	1.7181919E-6	70.0	70
GTATGCC	35	1.4188117E-9	70.0	42
TGCCGTC	35	1.4188117E-9	70.0	45
TATGCCG	35	1.4188117E-9	70.0	43
CCGTCTT	30	5.0224116E-8	70.0	47
ATGCCGT	35	1.4188117E-9	70.0	44
CGTATGC	40	3.8198777E-11	70.0	41
GCCGTCT	30	5.0224116E-8	70.0	46
ACAAAGT	45	1.10958354E-10	62.222225	29
TCTGCTT	45	1.10958354E-10	62.222225	53
TGCTTGA	45	1.10958354E-10	62.222225	55
CAAAGTG	45	1.10958354E-10	62.222225	30
GCTTGAA	45	1.10958354E-10	62.222225	56
CCGAGCC	45	1.10958354E-10	62.222225	14
CTGCTTG	45	1.10958354E-10	62.222225	54
TTGAAAA	45	1.10958354E-10	62.222225	58
TCTCCGA	45	1.10958354E-10	62.222225	11
CTTGAAA	45	1.10958354E-10	62.222225	57
AAAGTGA	45	1.10958354E-10	62.222225	31
TCCGAGC	45	1.10958354E-10	62.222225	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content