FastQCFastQC Report
Wed 25 May 2016
SRR1779658_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779658_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972505
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG187561.9286276163104559No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1500.032.66628354
GCCGTAA500.007210245427.99967213
CGTATCA2108.367351E-1121.66641256
CGCCGTA2151.1459633E-1021.1625453
CCGTATC2201.5643309E-1020.68157655
TCTCGGT2504.1836756E-1119.59977143
TTCCGAC900.00527508319.44521763
TGGTCGC2353.783498E-1019.36147349
GTAGATC2900.019.31011838
CCCGGTA950.00720817518.42178332
TCGGTGG2952.3646862E-1117.79640245
AGTGCGA1000.00968998617.49979422
CTAGTAC1452.4390547E-416.8963533
TATGCAG17650.016.85532670
CGGTGGT3301.2551027E-1015.90890446
CCTATAC2704.395406E-815.5553743
GGTCGCC3855.456968E-1215.45436450
GTGGTCG3201.382432E-915.3123248
TCGCCGT3009.733412E-915.16648952
ACCCTAC1859.03297E-515.1349566
Produced by FastQC (version 0.11.5)