FastQCFastQC Report
Wed 25 May 2016
SRR1779658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences972505
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT70210.7219500156811532No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT26330.27074410928478515No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG17300.17789111624104761No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14790.15208148030087248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG14450.051.0978870
TCGTATG20450.048.26760540
TATGCCG19250.048.03069743
CGTATGC19700.047.46241
CTCGTAT20000.047.42360739
ATCTCGT20050.047.2980537
TCTCGTA20150.047.06815338
AATCTCG19900.046.77292336
ATGCCGT19650.046.34243844
CGTCTTC20150.046.247348
CCGTCTT19700.045.7008647
GCCGTCT19900.045.59362446
GTATGCC20150.045.53544642
CGTATGA1250.044.8240441
TGCCGTC20550.044.1469545
CGTCAAT22050.043.79922532
GTCAATC21100.043.449533
CTGCTTG22100.042.14930354
TCGTCAA24300.040.3197431
GTCTTCT23100.040.1813349