FastQCFastQC Report
Wed 25 May 2016
SRR1779657_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779657_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336844
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157164.665661255655437No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7290.21642065763380083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1550.042.9019554
CGTATCA1550.040.64395556
GTCATAC350.001254688639.998811
TTACTCG350.001254688639.9988119
TAGATCT1950.037.69678539
GCAAAGT400.002417366334.9989646
GTTAGTC400.002417366334.998969
ACCGTAT400.002417366334.998968
CATTAAA1950.034.1015561
ATCATTA2000.033.24901259
GTATCAT1900.033.1569157
TCATTAA2000.031.49906560
CCGTATC2000.031.49906555
AACCGTA450.00430480631.1101867
TGTACTA802.4623423E-630.624095
AGATCTC2450.030.00356140
TTGTACT703.484303E-529.9991074
TTCATAC703.484303E-529.9991073
ACGGTTA604.9777667E-429.16580250
CGCCGTA2550.028.82267453
Produced by FastQC (version 0.11.5)