FastQCFastQC Report
Wed 25 May 2016
SRR1779657_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779657_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336844
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT57021.692771728159029No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT23210.6890429991331299No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG14650.43491942857821425No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13390.3975133889871869No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC5820.17278027811093563No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAAA150.002225275269.986261
AGGGGGG12000.053.94769370
TCGTATG18650.049.3614940
CTTGAAA18100.049.31431257
GTATGCC17750.048.94989842
CTCGTAT18750.048.9115439
TATGCCG17750.048.75251843
GTATCTC19100.048.73384535
CGTATGC18350.048.65690241
GCTTGAA18600.048.36503656
TCTCGTA19500.048.1073638
CGTATCT19550.047.97007434
TATCTCG19500.047.9207436
CCGTCTT16750.047.89107547
TGAAAAA20200.047.65326759
GTCTTCT17050.047.6364649
TCTTCTG17100.047.49716650
ATCTCGT19750.047.31414437
TTGAAAA19150.047.15874558
ACGTATC19900.046.95053533
Produced by FastQC (version 0.11.5)