FastQCFastQC Report
Wed 25 May 2016
SRR1779656_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779656_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188806
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120026.356789508807983No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG4480.23728059489634862No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAAC200.006943085752.50000441
TTCAACG200.006943085752.5000042
CTCTGAG200.006943085752.5000042
CCTATTC200.006943085752.5000042
GTAGTCA200.006943085752.50000439
ACGTGCA305.866859E-446.6666642
ACCCGTG350.001253105440.013
CGTATCA851.05699655E-732.94117756
CATGCAC450.00429943531.1111093
GTATCAT901.7455386E-731.11110957
TTCTGCA450.00429943531.1111092
TCATTAA802.4542114E-630.62500260
CATTAAA802.4542114E-630.62500261
ATCATTA802.4542114E-630.62500259
ATTAAAA853.9359966E-628.8235362
GACAGGT500.00719535627.9999987
GTACAAG500.00719535627.9999983
CTCCAGA500.00719535627.9999981
GCTCTGA500.00719535627.9999981
CTCTGGA500.00719535627.9999982
Produced by FastQC (version 0.11.5)