FastQCFastQC Report
Wed 25 May 2016
SRR1779656_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779656_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188806
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT43592.3087190025740707No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT16650.88185756808576No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG11080.5868457570204336No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9170.4856837176784636No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC3940.20867980890437807No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2290.12128851837335677No Hit
CATAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2170.11493278815291888No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT2020.10698812537737148No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA150.00221245870.0761148
CTAATGA150.00221245870.0761151
ATCTAGT252.8358136E-669.98331537
CATTATA150.002224119169.9833152
AGGGGGG7350.060.46177770
CATAAAC1000.059.485821
TCTAGTA308.364919E-658.3194338
GTATTAT252.3731215E-456.06088349
TCTGATT252.376234E-456.04602453
TGCTAGA1000.056.01632355
ATAAAAC252.3887164E-455.9866521
AAAACAC1450.055.504013
TAAAACA700.054.9868932
GATCTAG452.6924681E-854.43147336
TTATGCT407.395065E-752.55708352
TAATGAT200.006913300652.55708352
AGATAGT200.00692781752.5292254
TTTCCGT200.00692781752.5292254
CTGCTAG1000.052.52921754
TGTAGTG200.00694235652.50139241