FastQCFastQC Report
Wed 25 May 2016
SRR1779655_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779655_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355477
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149274.199146498929608No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7150.20113818896862526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTT200.00694849252.49926426
TCATTAA1450.038.6255860
ATCATTA1400.037.5047559
CGTATCA1550.036.12852556
TATCATT1400.035.00443358
ATTAAAA1500.035.00443362
GATCTTT501.7082534E-434.99950422
GTATCAT1550.033.8752657
CATTAAA1600.032.81665861
ATCTTTG552.9895725E-431.81773423
GTACCTC450.004304793731.1106746
TGTGTTT703.4844677E-529.99957832
GTAACAA703.4844677E-529.99957850
TAGATCT2000.029.7495839
TGTCAGA500.00720427127.99960527
GTATTGA500.00720427127.99960516
TTACGCC500.00720427127.9996054
ATAAACT500.00720427127.9996054
CGTTTTG500.00720427127.99960534
GCCGTAT2200.027.04507454