FastQCFastQC Report
Wed 25 May 2016
SRR1779655_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779655_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences355477
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT56001.5753480534605613No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT22170.6236690418789401No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG14540.4090278695949386No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12470.3507962540473786No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC6010.16906860359460668No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTA150.00222608469.9805915
CTTTATA501.4551915E-1162.982532
AGGGGGG9800.058.19814370
CTTATAT200.006955732652.485443
GTCACTA200.006955732652.4854463
CCTTTAT200.006955732652.485441
TTTATAC608.367351E-1152.485443
TCGTATG18200.051.72369840
GTATGCC17400.051.12812842
CGTATGC18150.050.74501441
CTTGAAA18050.050.027957
CTCGTAT18550.049.99316439
ATATACA5400.049.8935662
TGAAAAA19550.049.7700259
TTGAAAA18650.049.73210558
GAAAAAA20900.049.56957660
TCTGCTT17050.049.1101553
GTCTTCT17800.049.0022449
TCTTCTG17600.048.76295550
CCGTCTT17550.048.71600347
Produced by FastQC (version 0.11.5)