FastQCFastQC Report
Wed 25 May 2016
SRR1779654_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779654_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences563059
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151572.69190262476934No Hit
CTTATACACATCTGACGCTGCCGACGATATGCAGTGTGTAGATCTCGGTG7510.1333785624597067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA1700.041.17610556
CCGTATC1950.034.1022655
GTATCAT2150.032.5607457
ATCATTA2350.031.27909559
TTCTAAG450.004306831.1108362
AACCGTA450.004306831.1108367
GGGTAGG901.7561433E-731.1108361
ATACGAC450.004306831.1108363
TATCATT2400.030.62744958
CATTAAA2400.030.62744961
GCCGTAT2200.030.22700354
TAACGAA500.007207607427.9997514
TCATTAA2700.027.224460
TAGATCT2500.026.59976439
ACCTAAG700.001225840624.999781
CTATACG851.3079404E-424.7056644
GTCTAAT851.3079404E-424.70566448
ATTAAAA3200.024.06442362
TGTAGAT3100.023.70946737
GTTTAGG750.001831969223.3331281
Produced by FastQC (version 0.11.5)